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ISEV2016 Education Day
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Pre-Meeting Education Day

3rd May, 2016

The Pre-meeting Education Day is a successful tradition of the annual ISEV meetings. Focusing on particular topics within the extracellular vesicle field, select experts provide deep insight into certain basic aspects of extracellular vesicles. These educational presentations collectively illuminate burning questions and provide a comprehensive insight into the topic.

Encouraged by the very positive feedback from previous years, ISEV2016 for the first time provides two simultaneous Education Day programs:

(1) Novel developments of isolation and characterization of extracellular vesicles
(2) Extracellular vesicle therapeutics

Education Day programs provide unique learning opportunities in the rapidly emerging field of extracellular vesicles. They provide solid background information for attendees of ISEV2016 before visiting the Annual Meeting’s symposium and poster sessions.


Education Day 1st Sessions

Novel developments of isolation and characterization of extracellular vesicles

8:15 Registration
9:00 Welcome

• Cecilia LasserMassive Online Open Course MOOC - basics of extracellular vesicles

Introduction to EVs and EV isolation
• Jan Lötvall: Heterogeneity and preanalytical factors
• An Hendrix: Isolation techniques - comparative study
• Chris Gardiner: Summary of the results of the ISEV survey

EV imaging
• Guillaume van Niel: Transmission electron microscopy of EVs
• Alain Brisson: Cryo electron microscopy of EVs
• Charles Lai: Visualization and tracking of EVs


High throughput analysis of EVs
• Chris Gardiner: NTA analysis of EVs
• Edwin van der Pol: Tunable resistive pulse sensing analysis of EVs
• Marca Wauben: Flow cytometry of EVs
• Ester Nolte-’t Hoen: Comparison of NTA/qNano/high resolution flow cytometry

Lunch break

Novel developments in EV isolation
• Jaesung Park: Aqueous two phase system
• Leonora Balaj: Heparin affinity isolation of EVs
• Anirban Ghosh: Synthetic peptide-based affinity isolation of EVs

Novel developments of EV analysis
• Fredrik Höök: Surface plasmon resonance analysis of EVs
• Sergei Kruglik : Raman tweezer nanospectroscopy of EVs
• Maiwenn Kersaudy-Kerhoas: Microfluidics analysis of EVs


OMICS Technologies
• Andrew Hill: Transcriptomics of EVs
• Suresh Mathivanan: Integrative proteogenomics and shotgun lipidomics of EVs
• Juan Manuel Falcon-Perez: Metabolomics of EVs

EV enrichment in clinical settings
• Edit I Buzás: Lipid determination of EV preparations
• Rienk Nieuwland: Size exclusion chromatography of EVs
• Samir El Andaloussi: Purification of EVs using ultrafiltration and subsequent size exclusion chromatography
• Harry Holthofer: Hydrostatic dialysis of EVs

Concluding remarks: María Yáñez-Mó

Education Day 2nd Sessions

Extracellular vesicle therapeutics

8.15 Registration
9:00 Welcome

• Yong Song Gho: Massive Online Open Course (MOOC) - basics of extracellular vesicles

Introduction to EV therapeutics
• Bernd Giebel: EV-based therapeutics; general aspects and perspectives
• Eva Rohde: Manufacturing, safety and regulatory requirements

EV-based immunomodulation
• Paul D Robbins: Immunomodulation by immune cell-derived EVs
• Michael Graner: Immunomodulation by tumor EVs
• Giovanni Camussi: Immunomodulation by stem cell EVs
• Susanne Gabrielsson: Immunomodulation by milk EVs


Design of EV-based or EV-inspired carriers
• Yong Song Gho: Bioinspired exosome-mimetic nanovesicles
• Hang Hubert Yin: Considerations regarding membrane curvature and targeting
• Casey Maguire: Vexosomes
• Raymond Schiffelers: Artificial bioinspired EVs

Lunch break

EV vaccines in infectious diseases
• Ken Witwer: Challenges of anti-viral EV vaccine development
• Oh Youn Kim: OMV-based vaccination
• Hernando del Portillo: Vaccination with parasite derived EVs


EV therapeutics in clinic
• Clotilde Thery: EV therapy in cancer
• Lawrence Rajendran: EV therapy in neurological diseases
• Sai Kiang Lim: MSC-derived EV therapy

EVs in preclinical models
• Susmita Sahoo: Cardiac repair after myocardial infarction

Plant EV therapeutics
• Riccardo Alessandro: Therapeutic applications of edible plant EVs

Concluding remarks: Lorraine O’Driscoll